El Tigre: The Adventures of Manny Rivera episode The Mother of All Tigres
April 07, 2007
At the end of the first segment of the fifth episode of El Tigre, various random villains attack the city, and among them are "The Zombie Plumbers". They come out of a manhole, were two plumbers, looking like the Mario Brothers, and both with an "M" on their hat, were working. The Zombie plumbers themselves are instead parodies of Wario, sincethey wear similar clothes, with a "W" on their hat, and one of them has zigzag shaped mustache like Wario's.
The creators of El Tigre held no right over Nintendo's Mario, but they only icluded a minor reference to the series.