Super Robot Monkey Team Hyperforce Go! episode Galactic Smash: Space Attack (Part 1)
September 9, 2006
At one point in the first episode of season 4 of the series (Episode 40), Otto gets a hologram projector and starts using it on Chiro turning him in forms similar to Mario, Link from Zelda and Donkey Kong.
His incarnation of Mario is specifically based on his jumping sprites from Super Mario Bros., has a mustache and looks pixelated, but it retains Chiro's clothes and hairstyle.
The Answer Studio, that created Super Robot Monkey Team Hyperforce Go! didn't held any right over Nintendo's Mario, but they only included a minor reference.