Uncle Grandpa episode Afraid of the Dark
October 21, 2013
In a scene in the eleventh episode of Uncle Grandpa, the titular character looks closely at a photo of a knight and a motorcycle to reveal a map of his current location. The map is heavily based on Super Mario World's map, containing what seems to be a Warp Pipe and a Toad House amongst the various parts of the room he's in.
The creators of Uncle Grandpa held no right over Nintendo's Mario, but they only included a minor reference to the series.